Testing for albumin 1) The supernatant is separated into two parts, one to be used for part D and another for part E 2) One tube is put in a boiling water bath for 5 minutes. 3) The cloudiness of the heated supernatant is then compared with the unheated portion that was kept. (E) Testing for glucose 1) 10 drops of the Benedicts solution is added to the unheated supernatant from part D. 2) A second test tube is filled with similar amount of water and Benedicts solution (this is to serve as a control). 3) The tubes are put in a boiling water bath for 3 minutes and allowed to cool. 4) The colour of the samples were observed.
RESULTS : Test (A) Observation of urine; The urine smelled nutty * The colour hue is pale yellow * The clarity of the urine is clear Test (B) Test for pH; * The pH of the urine was 6. 00 Test (D) Testing for albumin; * The urine turned cloudy after being heated, the initial colour which was bluish yellow turned dark yellow green in colour, protein was not present. Test (E) Testing for glucose * Initial colour of the solution which was bluish yellow turned to light green with participate, reducing sugar is present. DISCUSSION The colour of urine can stretch from the almost clear to a dark orange and it’s simply matter of hydration.Food can also have an effect on colour and can cause different smells, as can any medications or supplements. The pH level of normal urine should be slightly below 7 because of the presence of urea in the urine.
A diet high in citrus, vegetables, or dairy can increase urine PH. Protein is usually not found in the urine. The presence of albumin in the urine (albumin’s) NAS been shown to be a sensitive indicator tot with diabetes and with hypertension. Kidney disease in people Normally, urine contains low levels or no glucose because the kidneys are able to claim all of the filtered glucose back into the bloodstream.High levels of glucose present in urine could mean that the donor could be suffering from diabetes. The presence of amorphous phosphate and oxalate crystals are normal in urine, but if there are any abnormalities, it could be a sign that the donor could be abusing the use of drugs. 1) What can you conclude about the health status and health status of the urine donor? The results show that the donor is a healthy individual, and the urine status is normal.
) Explain briefly three possibilities of disease or abnormal conditions that can be detected from the urine analysis. I) Dehydration could be detected by the observation of urine test from the urine analysis. It) Kidney failure could be detected by the testing for albumin from the urine analysis. Iii) Diabetes could be detected by the testing of glucose from the urine analysis