Susceptibility Lab #4 Purpose: The lab was completed to determine the susceptibility of Staphylococcus epidermis, E. Coli, Pseudonymous organisms, Staphylococcus erasures, and Candida Albanians against the antibiotics Streptomycin, Tetracycline, Penicillin, Geocentric, Chlorination, Erythrocytes, and Inclining using Kirby-Bauer Method/ Disc Diffusion testing. Materials: 5 Mueller-Hint media plates Tropic Soy Broth (TTS) containing Stash epidermis TTS containing E. Coli TTS containing Pseudonymous organisms TTS containing Staphylococcus erasures
TTS containing Candida Albanians Black sharpie 5 Sterile swabs Incubator Bizarre bag KBPS zone size interpretation Ruler Disc dispenser Antibiotics -Streptomycin (S) -Tetracycline (ET) -Penicillin (P) -Geocentric (CNN) -Chlorination (C) -Inclining (AMP) -Erythrocytes (E) Procedure: In a lab group of four, all of the materials listed above were gathered. The group labeled all of the media plates with the respected organisms. The TTS broth was properly mixed using a swirling technique.
Using a sterile swab, each plate was inoculated with the matching organism. The plates were inoculated using an even lawn streak by covering the entire innocuous. After each plate was separately inoculated, the seven antibiotics listed above were administered onto each plate using the disc dispenser. The antibiotics were properly placed and in full contact, touching each one on all of the media plates. The media plates were covered and placed in the incubator at ICC for more than 24 hours. The contaminated swabs were placed in the bizarre bag for disposal.
Tater incubation period was completed, the group measured the diameter (mm) to the inhibition zone on each media plate and recorded the data. The KBPS zone size interpretation chart was used to determine the results of susceptibility. Results: Antibiotic vs.. Microbes Diameter Antibiotic Stash pep E. Coli Pseudonymous organisms Stash erasures Candida Albanians s 12 8 14 6 24 16 28 CNN c 20 AMP 13 26 Conclusion: The results were visually analyzed and recorded above. After obtaining the above results, we compared them to the KBPS zone size interpretation chart given.
We were able to determine, for each organism, if it was sensitive, intermediate, or resistant to the corresponding antibiotic. Looking at the organism Stash pep, we found that it was resistant to Streptomycin, Tetracycline, Penicillin, and Inclining. Therefore, those antibiotics were undesirable for treatment. Stash pep was sensitive to Geocentric, Chlorination, and Erythrocytes. Thus, they were desirable antibiotic treatments. The best antibiotic to treat Stash pep, however, would be Chlorination because it has the highest sensitivity.
By observing E. Coli, it was determined to be resistant to Streptomycin, Inclining, and Erythrocytes. E. Coli was only sensitive to Tetracycline, Geocentric, and Chlorination. The best antibiotic to treat E. Coli would be Tetracycline; the next best antibiotic would be Chlorination. This is due to the highest sensitivity records. When observing Pseudonymous organisms, resistance to Streptomycin, Chlorination, and Inclining was determined. Pseudonymous organisms was sensitive to Tetracycline and Geocentric.
The best antibiotic to treat Pseudonymous erogenous would be Geocentric due to the high sensitivity. Looking at Stash erasures, it was determined to be resistant to Streptomycin. It was only sensitive to Tetracycline, Penicillin, Geocentric, Chlorination, and Erythrocytes. It showed intermediate tendencies towards Inclining. The best antibiotic to treat Stash erasures was with Tetracycline due to the high sensitivity. Candida Albanian demonstrated resistance towards Streptomycin, Tetracycline, Geocentric, Chlorination, and Inclining. No sensitivity was observed; therefore,